HALO® ELEVATE – Engineered for High pH Performance Across the Range
Built upon proven Fused-Core® particle technology for speed and efficiency, the HALO® ELEVATE product family incorporates surface modified organo-silane technology for alkaline resistance resulting in excellent stability in high pH environments.
With a wide operational use range of pH 2-12, HALO® Elevate allows for robust method development and improved separations for basic compounds that may present problems such as poor peak shapes, inadequate retention or limited load tolerance at low pH. Ideal for use with high pH mobile phases.
FEATURES OF HALO® ELEVATE
Three Phase Offerings: C18, C8 and PHENYL-HEXYL
Excellent stability for high pH, high temperature environments
Three Particle Sizes: 2, 2.7 and 5 µm
Robust Column Option to work the full range of operating conditions for separation selectivity of acids, bases, neutrals and zwitterions
Proven HALO® Fused-Core® technology for uniform column loading, quality separations, speed and ruggedness
PRODUCT CHARACTERISTICS
C18
Ligand: dimethyloctadecyl
Particle Size: 2, 2.7 and 5µm
Pore Size: 120 Å
USP Designation: L1
*Reported: 2, 2.7, 5µm respectively
Carbon Load*: 5.2%, 5.6%, 4.5%
Surface Area*: 70, 75, 60 m2/g
Endcapped: YES
pH Operating Range: 2-12
Temp Limit: 60 °C
Pressure Stability*: 1000, 600, 600
C8
Ligand: dimethyloctyl
Particle Size: 2, 2.7 and 5µm
Pore Size: 120 Å
USP Designation: L7
*Reported: 2, 2.7, 5µm respectively
Carbon Load*: 3.8%, 4.2%, 3.2%
Surface Area*: 70, 75, 60 m2/g
Endcapped: YES
pH Operating Range: 2-12
Temp Limit: 60 °C
Pressure Stability*: 1000, 600, 600
PHENYL-HExyl
Ligand: dimethylphenylhexyl
Particle Size: 2, 2.7 and 5µm
Pore Size: 120 Å
USP Designation: L11
*Reported: 2, 2.7, 5µm respectively
Carbon Load: 4.5%, 5.1%, 4.0%
Surface Area*: 70, 75, 60 m2/g
Endcapped: YES
pH Operating Range: 2-12
Temp Limit: 60 °C
Pressure Stability*: 1000, 600, 600
HALO® Advantage!
HIGH pH STABILITY TESTING @ 50 °C
A combination of neutrals and bases are observed on a HALO® ELEVATE C18, C8, and Phenyl-Hexyl column showing excellent reproducibility over 9,000 column volumes. Separation is performed under high temperature (50 °C), and high pH (10), isocratic conditions.
The chromatograms that were included in the stability run above. A chromatogram from beginning and end of the run can be found below for each product chemistry all demonstrating excellent stability of performance over time.
PEAK IDENTITIES
- Related Compound F & G
- Related Compound B
- Related Compound E
- Related Compound A
- Impurity B
- Omeprazole
- Impurity H
- N’-Methyl Omeprazole
- Impurity C
Modernized Omeprazole Separation on HALO® Elevate C18
Run Conditions: pH 10.7, @ 60 °C
*see toss sheet for full test conditions
PEAK IDENTITIES
- Butyl Paraben (neutral)
- Doxylamine (base)
- Chlorpheniramine (base)
- Doxepin (base)
- Amitriptyline (base)
- Trimipramine (base)
Lot to Lot Study of HALO® Elevate C18
Run Conditions: pH 10 @ 60°C
The average %RSD across all of the compounds was < 0.6%.
*see toss sheet for full test conditions
Featured Documents
SHOP HALO® ELEVATE
HALO 120 Å ELEVATE C18
Price range: $645.00 through $1,300.00
Description
- Surface modified organo-silane technology for alkaline resistance
- Excellent stability under high pH conditions
- Improved peak shape and loading of basic compounds
- Wide operational use range of pH 2-12 for robust method development
- USP: L1
- Available particle size: 2 and 2.7 um
- Ligand: dimethyloctadecylsilane
- Chemical Classification: alkyl
- USP: L1
- Pore Size: 120 Å
- Low pH/T Limit: 2/60 °C
- High pH/T Limit: 12/60 °C
- % Carbon Load: 5.6
- Endcapped: Yes
HALO® Method Validation Kits Available
Choose three columns of the same part number for method validation and receive 15% off your set. Each column will be from a different lot.
Enter code “VALIDATE” at checkout for promotion to apply. Learn More.
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